Data collection and processing

Data on routine resistance tests were received annually from fifteen participating virology laboratories in the UK, and imported into a central SQL database. The nucleotide sequences were then processed through the Stanford University Genotypic Resistance Interpretation Algorithm (HIVdb) to obtain aligned sequences, amino acid mutations and drug susceptibility data. These were stored in the database along with subtype data from the Rega Institute.

Where possible, tests were linked (using pseudo-anonymised patient identifiers) to UKCHIC clinical data, the UK Seroconverter Register, CHIPS and to surveillance data at Public Health England. After extensive data cleaning, a final dataset was created for analysis.  Relevant variables can be made available to collaborators.