Data on routine resistance tests are received annually from fifteen participating virology laboratories in the UK, and imported into a central SQL database. The nucleotide sequences are then processed through the Stanford University Genotypic Resistance Interpretation Algorithm (HIVdb) to obtain aligned sequences, amino acid mutations and drug susceptibility data. These are stored in the database along with subtype data from the Rega Institute.
Where possible, tests are linked (using pseudo-anonymised patient identifiers) to UKCHIC clinical data, the UK Seroconverter Register, CHIPS and to surveillance data at Public Health England. After extensive data cleaning, a final dataset is created for analysis. Relevant variables from this are made available to collaborators.